Plasmid_Backbone
Part:BBa_K4284011
Designed by: Zhang Xinchi Group: iGEM22_PINGHE (2022-08-11)
pET28a-EGFP-backbone
pET28a-EGFP-backbone
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 192
Illegal XbaI site found at 245 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 192
Illegal NotI site found at 165 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 192
Illegal BglII site found at 311
Illegal BamHI site found at 198
Illegal XhoI site found at 158 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 192
Illegal XbaI site found at 245 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 192
Illegal XbaI site found at 245
Illegal NgoMIV site found at 343
Illegal NgoMIV site found at 1931
Illegal NgoMIV site found at 2091
Illegal NgoMIV site found at 4322 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI site found at 3011
[edit]
Categories
Parameters
None |